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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: Exposure to Concentrated Ambient Fine Particulate Matter Induces Vascular Endothelial Dysfunction via miR-21
doi: 10.7150/ijbs.19868
Figure Lengend Snippet: miR-21 is involved in the PM 2.5 -induced increase in vascular endothelial cell permeability. Total RNAs were extracted from NS- or PM 2.5 -treated HUVECs and aortic arches of SD rats. (A) Affymetrix GeneChip miRNA 1.0 Array analysis of the differential expression miRNAs in NS or PM 2.5 treated HUVECs. Hierarchical clustering was performed using 84 miRNAs selected from among 2,536 miRNAs. Each row corresponds to one miRNA, and the two columns correspond to the NC and PM 2.5 groups. (B) Volcano plot of miRNA analysis. Plots of log P value versus log 2 fold change for HUVECs treated and untreated with PM 2.5 . The two vertical lines in each panel mark twofold changes. Red spots indicate miRNAs with highly significant fold changes. Labels indicate miR-21. (C) miR-21 expression was verified using qRT-PCR from HUVECs treated or untreated with PM 2.5 (80 µg/mL) for 24 h. (D) The expression of miR-21 in the aortic arches of rats treated with NS or PM 2.5 was measured using qRT-PCR. (E) Effect of PM 2.5 on HUVEC monolayer permeability. Cells were cultured until confluent in transwell inserts and incubated with or without the miR-21 inhibitor for 24 h and then stimulated with PM 2.5 (80 µg/mL) or left untreated for 24 h, and then FITC-dextran was added to the top chamber. Samples were removed from the bottom chamber 24 h later and analyzed with a fluorometer.* P < 0.05, *** P < 0.001.
Article Snippet: The miRNA expression was analyzed using the Affymetrix
Techniques: Permeability, Expressing, Quantitative RT-PCR, Cell Culture, Incubation